For mRNA sequencing (denovo or reference), mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. Sequencing of the mRNA is the method of choice for analyzing the transcriptomes, e.g., of biological responses or of disease states. It is both a highly accurate and sensitive method for quantifying gene expression.
Sequencing of mRNA provides a detailed view of the mRNA molecules of known and novel transcript isoforms. Other features, like gene fusions, can also be detected. mRNA sequencing offers these analyses while requiring less sequencing capacity than total RNA sequencing.
mRNA sequencing allows for:
- Measuring differential gene expression
- Detecting previously unknown transcripts
- Capturing known and novel transcript isoforms