APTBIO offers iTRAQ protein quantification service suited for unbiased untargeted differentially expressed proteins discovery. Relative quantification of proteins for differentially expressed proteins discovery in complex mixtures by mass spectrometry can easily and quickly be achieved using iTRAQ technology. iTRAQ is ideally suited for comparing normal and treated samples, time course studies, biological replicates and provides relative quantitation.

iTRAQ is a liquid-based proteomics technique. It labels the Lysine residues and N terminus of all peptides with isotopes of identical masses, thus allows accurate quantitative comparisons between 2 (2-plex) to 8 (8-plex) samples. This approach can provide both protein identification and protein ratios in one experiment.


Bioinformatics analysis:

1) Protein GO analysis

2) Protein KEGG analysis

3) Differentially expressed proteins GO enrichment analysis

4) Differentially expressed proteins KEGG enrichment analysis

5) Differentially expressed proteins cluster analysis

6) Differentially expressed proteins PPI analysis

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