Glycopeptide analysis is a very convenient method to link the glycosylation information to exact locations (glycosylation sites) on proteins. It provides very helpful information about the glycans on one or more proteins.
The analysis of glycopeptides can be used to discover and characterize some novel and interesting molecules, which leads to the development of new products. Comparative glycopeptide analysis can be helpful both in diagnosing or treating disease and also in developing a better understanding of disease progression and pathology.
Currently glycopeptide analysis commonly employs a bottom-up approach. This method typically involves a combination of specific enzymatic proteolysis followed by fractionation of glycopeptides by affinity chromatography or liquid chromatography and eventually glycopeptide analysis by mass spectrometry.
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