At present, there are two types of mass spectrometry used for molecular weight analysis: Matrix-assisted laser desorption / ionization (MALDI-TOF) and Electrospray ionization (ESI-MS). The two methods have their own advantages and applications.

MALDI-TOF can be used to directly measure the molecular weight of biopharmaceuticals, while ESI-MS analyzes multiple charge signals obtained by electric spray, and speculates the precise molecular weight through deconvolution. MALDI-TOF-MS is more suitable for analyzing biopharmaceuticals with molecular weight less than 25kDa. ESI-MS is recommended for molecular weight identification of protein substances with molecular weight of larger than 25kDa.

APTBIO has established a platform with both MALDI-TOF and ESI-MS technologies, which can be used to determine the molecular weight of all kinds of biopharmaceuticals, including antibodies, vaccines, proteins drugs, and polypeptides.

MALDI-TOF-MS MW result

ESI-MS MW result